The Antioxidant Effect of Mitochondrially Targeted Antioxidant SkQ1 on the Isolated Rat Heart Model

Mitochondrially targeted antioxidants based on Skulachev ions (SkQ1) are extremely attractive for neutralizing reactive oxygen species directly in the mitochondrial matrix. The aim was to examine antioxidant and cardioprotective status of SkQ1 mitochondrially an isolated rat heart model ischemia reperfusion after cold cardioplegia. Material methods. effects different concentrations (1200 ng/ml, 120 12 ng/ml) were explored hearts Wistar rats ( n =50) during 240-min levels oxidative stress, changes myocardial damage markers (classical highly specific) cardiac function (coronary flow velocity, rate, systolic pressure) assessed. Results. use at ng/ml resulted a significant neutralization stress manifestations P <0.05). minimum concentration NO metabolites (nitrates nitrites) (36.2 [30.8; 39.8] µmol/ml) maintained pre-ischemic level throughout 30-minute period, while malonic dialdehyde (49.5 [41.1; 58.9] µmol/g) lower compared with dose. Due «mitigation» intracellular enzymes specific rose more slowly reperfusion, recovery occurred higher rate showed stability upon restoration perfusion. Conclusion. strong properties ex vivo study.